By W. N. Aldridge (eds.)
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Extra resources for A Symposium on Mechanisms of Toxicity
If a normal rat is exposed to cold, its body temperature decreases slightly. In earlier experiments (Meeter & Wolthuis, 1968) it was observed that when the hypothermia caused by anti-ChE is fully developed, exposure to cold induces a markedly greater fall in body temperature. If the anti-ChE's simply shift the SPHR to a lower level there would be no reason for a reduced ability to cope with cold. The cold-exposure experiments were repeated and two important factors of defence against cold were also studied-pilo-erection and shivering.
The residue was dissolved in 0·1 ml of water and aliquots of this solution were subjected to paper electrophoresis at pH 2·5 as described in the text. IDOl each, were added as markers to the sample. After electrophoresis, 1 em sections of the paper strip were cut out for determinations of radioactivity. The only radioactive components found are those shown in the figure. ) or by heating at I oooc for a few minutes. It is relatively stable at 26°C in strong acid solution. Methionine sulphoximine phosphate is about 50% hydrolysed when heated at 100°C in 1 NHCl for 85 min.
On the other hand, earlier studies (Pare & Sandler, 1959) indicate that the administration of 3,4-dihydroxyphenylalanine (dopa) does not have anti-depressant properties in man, suggesting that inhibition of the oxidation of dopamine may not be important. , 1969) and it is possible that this is the relevant amine. It is likely that the pharmacological actions in experimental animals and the clinical actions of these drugs in man are due to two different mechanisms and suggests that care must be exercised in attempting to extrapolate to man data obtained in experimental animals with substratespecific monoamine oxidase inhibitors.